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KMID : 0379119960240030206
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Korean Journal of Mycology
1996 Volume.24 No. 3 p.206 ~ p.213
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Isolation of a Thermophilic Mutant , Talaromyces luteus 2004 in relation to the Regulation of Carboxymethylcellulase Production and Enzymatic characteristics
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Abstract
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Talaromyces luteus 2004, a thermophilic mutant of T. luteus 6112 was obtained by mutagenesis with N-methyl-N´-nitro-N-nitrosoguanidine. T. luteus 2004 produced thermophilic carboxymethylcellulase (CMCsse), and other polysaccharide enzymes: avicellase, xylanase, and β-glucosidase. Induction of CMCase production was shown at the highest level in 3% carboxymethylcellulose (CMC) minimal broth, indicating that CMC could work as an inducer. However, glucose and D-cellobiose showed catabolite repression for CMCase production which was under the control of CMC utilization. Optimal conditions for CMCase activity were at 70℃ and pH 4.0, suggesting that CMCase of T. luteus 2004 was a thermophilic enzyme.
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